Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3)

被引：15

作者：

Zheng, Hongchen ^{[1
]}

Yu, Zhenxiao ^{[1
]}

Shu, Wenju ^{[1
]}

Fu, Xiaoping ^{[1
]}

Zhao, Xingya ^{[1
]}

Yang, Shibin ^{[1
]}

Tan, Ming ^{[1
]}

Xu, Jianyong ^{[1
]}

Liu, Yihan ^{[2
]}

Song, Hui ^{[3
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China

[2] Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China

[3] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2019年 / 103卷 / 03期

关键词：

Ethanol treatment; Escherichia coli; Heterologous protein expression; Maltose ABC transporter; prpD; EXTRACELLULAR PRODUCTION; SOLUBLE EXPRESSION; BINDING; IDENTIFICATION; PURIFICATION; TRANSPORTER; TOLERANCE; SUBUNIT; MODE;

D O I：

10.1007/s00253-018-9509-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method involving ethanol-induced increase in the heterologous recombinant protein expression in E. coli cells was commonly used in recent studies. However, the detailed mechanism of this method is still to be revealed. This work used comparative transcriptomic analysis and numerous experiments to uncover the mechanism of ethanol effects on the expression of heterologous catalase in the recombinant strain E. coli BL21 (pET26b-katA). The key regulatory genes malK and prpD were found to have the most significant effects on the expression of heterologous catalase. Thus, the maltose ABC transporter and carbon metabolism from propanoate metabolism to citrate cycle were found to be the main regulatory pathways activated by ethanol to enhance the synthesis of heterologous proteins. Based on these mechanisms, a universally applicable E. coli expression host strain for improving the expression of heterologous proteins might be constructed.

引用

页码：1441 / 1453

页数：13

共 27 条

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