AIS is an oncogene amplified in squamous cell carcinoma

被引：418

作者：

Hibi, K

Trink, B

Patturajan, M

Westra, WH

Caballero, OL

Hill, DE

Ratovitski, EA

Jen, J

Sidransky, D

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Div Head & Neck Canc Res, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[3] Oncogene Res Prod, Cambridge, MA 02142 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 10期

关键词：

p63 tumor-suppressor gene;

D O I：

10.1073/pnas.97.10.5462

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We and others recently isolated a human p53 homologue (p40/p51/p63/p73L) and localized the gene to the distal long arm of chromosome 3. Here we sought to examine the role of p40/p73L, two variants lacking the N-terminal transactivation domain, in cancer. Fluorescent in situ hybridization (FISH) analysis revealed frequent amplification of this gene locus in primary squamous cell carcinoma of the lung and head and neck cancer cell lines. (We named this locus AIS for amplified In squamous cell carcinoma.) Furthermore, amplification of the AIS locus was accompanied by RNA and protein overexpression of a variant p68(AIS) lacking the terminal transactivation domain. Protein overexpression in primary lung tumors was limited to squamous cell carcinoma and tumors known to harbor a high frequency of p53 mutations. Overexpression of p40(AIS) in Rat 1a cells led to an increase in soft agar growth and tumor size in mice. Our results support the idea that AIS plays an oncogenic role in human cancer.

引用

页码：5462 / 5467

页数：6

共 29 条

[1]

Agami R, 1999, NATURE, V399, P809

[2]

Brass N, 1997, CANCER RES, V57, P2290

[3]

Gong JG, 1999, NATURE, V399, P806

[4]

Hagiwara K, 1999, CANCER RES, V59, P4165

[5] A simplified system for generating recombinant adenoviruses [J].

He, TC ;

Zhou, SB ;

da Costa, LT ;

Yu, J ;

Kinzler, KW ;

Vogelstein, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2509-2514

[6]

Hibi K, 1998, CANCER RES, V58, P1405

[7]

Hibi K, 1996, CANCER RES, V56, P480

[8] PARTICIPATION OF CYCLIN-A IN MYC-INDUCED APOPTOSIS [J].

HOANG, AT ;

COHEN, KJ ;

BARRETT, JF ;

BERGSTROM, DA ;

DANG, CV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :6875-6879

[9] P53 MUTATIONS IN HUMAN CANCERS [J].

HOLLSTEIN, M ;

SIDRANSKY, D ;

VOGELSTEIN, B ;

HARRIS, CC .

SCIENCE, 1991, 253 (5015) :49-53

[10] Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers [J].

Kaghad, M ;

Bonnet, H ;

Yang, A ;

Creancier, L ;

Biscan, JC ;

Valent, A ;

Minty, A ;

Chalon, P ;

Lelias, JM ;

Dumont, X ;

Ferrara, P ;

McKeon, F ;

Caput, D .

CELL, 1997, 90 (04) :809-819

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