Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding

被引：1158

作者：

Garrus, JE

von Schwedler, UK

Pornillos, OW

Morham, SG

Zavitz, KH

Wang, HE

Wettstein, DA

Stray, KM

Côté, M

Rich, RL

Myszka, DG

Sundquist, WI ^{[1
]}

机构：

[1] Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84132 USA

[2] Univ Utah, Sch Med, Ctr Biomol Interact Anal, Salt Lake City, UT 84132 USA

[3] Myriad Genet, Salt Lake City, UT 84108 USA

来源：

CELL | 2001年 / 107卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(01)00506-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Like other enveloped viruses, HIV-1 uses cellular machinery to bud from infected cells. We now show that Tsg101 protein, which functions in vacuolar protein sorting (Vps), is required for HIV-1 budding. The UEV domain of Tsg101 binds to an essential tetrapeptide (PTAP) motif within the p6 domain of the structural Gag protein and also to ubiquitin. Depletion of cellular Tsg101 by small interfering RNA arrests HIV-1 budding at a late stage, and budding is rescued by reintroduction of Tsg101. Dominant negative mutant Vps4 proteins that inhibit vacuolar protein sorting also arrest HIV-1 and MLV budding. These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies.

引用

页码：55 / 65

页数：11

共 60 条

[1] The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways [J].