Energy-Independent Intracellular Gene Delivery Mediated by Polymeric Biomimetics of Cell-Penetrating Peptides

被引：41

作者：

Chae, Su Young ^{[1
]}

Kim, Hyun June ^{[1
]}

Lee, Min Sang ^{[1
]}

Jang, Yeon Lim ^{[1
]}

Lee, Yuhan ^{[2
]}

Lee, Soo Hyeon ^{[2
]}

Lee, Kyuri ^{[1
]}

Kim, Sun Hwa ^{[3
]}

Kim, Hong Tae ^{[4
]}

Chi, Sang-Cheol ^{[1
]}

Park, Tae Gwan ^{[2
]}

Jeong, Ji Hoon ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Sch Pharm, Suwon 440746, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[3] Yonsei Univ Hlth Syst, Yonsei Integrat Res Inst Cerebral & Cardiovasc Di, Seoul 120752, South Korea

[4] Sungkyunkwan Univ, Coll Nat Sci, Dept Biol Sci, Suwon 440746, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

amphiphiles; biomimetics; gene delivery; micelles; polymers; FACIAL AMPHIPHILES; LOW-TEMPERATURE; DNA COMPLEXES; POLYETHYLENIMINE; TRANSFECTION; MECHANISMS; SYSTEMS;

D O I：

10.1002/mabi.201100088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficient gene transfer into mammalian cells mediated by small molecular amphiphile-polymer conjugates, bile acid-polyethylenimine (BA-PEI), is demonstrated, opening an efficient transport route for genetic materials across the cell membrane. This process occurs without the aid of endocytosis or other energy-consuming processes, thus mimicking macromolecular transduction by cell-penetrating peptides. The exposure of a hydrophilic face of the amphiphilic BA moiety on the surface of BA-PEI/DNA complex that mediates direct contact of the BA molecules to the cell surface seems to play an important role in the endocytosis- and energy-independent internalization process. The new modality of the polymeric biomimetics can be applied to enhanced delivery of macromolecular therapeutics.

引用

页码：1169 / 1174

页数：6

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