In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides

被引：48

作者：

Brandén, LJ ^{[1
]}

Christensson, B

Smith, CIE

机构：

[1] Karolinska Inst, Novum, Dept Biosci, SE-14157 Huddinge, Sweden

[2] Huddinge Univ Hosp, Karolinska Inst, Clin Res Ctr, S-14186 Huddinge, Sweden

[3] Huddinge Univ Hosp, Dept Immunol Microbiol Pathol & Infect Dis, S-14186 Huddinge, Sweden

来源：

GENE THERAPY | 2001年 / 8卷 / 01期

关键词：

PNA; NLS; in vivo; transfection; gene therapy;

D O I：

10.1038/sj.gt.3301345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.

引用

页码：84 / 87

页数：4

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