Site-specific incorporation of a redox-active amino acid into proteins

被引:121
作者
Alfonta, L
Zhang, ZW
Uryu, S
Loo, JA
Schultz, PG
机构
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[3] Univ Calif Los Angeles, Dept Biochem, Los Angeles, CA 90095 USA
关键词
D O I
10.1021/ja038242x
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The redox-active amino acid 3,4-dihydroxy-L-phenylalanine (DHP), which can undergo two-electron oxidation to a quinone, has been incorporated selectively and efficiently into proteins in Escherichia coli in response to a TAG codon. We have demonstrated that DHP can be oxidized electrochemically within the protein. The ability to incorporate a redox-active amino acid site specifically into proteins should facilitate the study of electron transfer in proteins, as well as enable the engineering of redox proteins with novel properties. Copyright © 2003 American Chemical Society.
引用
收藏
页码:14662 / 14663
页数:2
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