Analysis of terpenelactones in Ginkgo biloba by high performance liquid chromatography and evaporative light scattering detection

被引：38

作者：

Ganzera, M

Zhao, JP

Khan, LA ^{[1
]}

机构：

[1] Univ Mississippi, Res Inst Pharmaceut Sci, Natl Ctr Nat Prod Res, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2001年 / 49卷 / 09期

关键词：

Ginkgo biloba; HPLC; evaporative light scattering detection; ginkgolide; bilobalide;

D O I：

10.1248/cpb.49.1170

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A reversed phase HPLC method permitting the determination of 5 terpenelactones in Ginkgo biloba, without the need of any sample preparation is presented in this paper. The compounds were successfully separated within 25 min by using a C-12 column, an evaporative light scattering (ELS) detector and a mobile phase comprising of ammonium acetate buffer, methanol and isobutanol. All terpenelactones were detectable at concentrations as low as 20.3 mug/ml. The analysis of G. biloba market products showed remarkable variations in the lactone content, and more than 2 fold differences in the suggested daily doses of the total lactones, from 8.84 mg to 18.28 mg, respectively.

引用

页码：1170 / 1173

页数：4

共 16 条

[1]

[Anonymous], PHARM PHARM LETT

[2]

Blumenthal M, 1999, HERBALGRAM, V47, P64

[3] EVAPORATIVE LIGHT-SCATTERING AND THERMOSPRAY MASS-SPECTROMETRY - 2 ALTERNATIVE METHODS FOR DETECTION AND QUANTITATIVE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GINKGOLIDES AND BILOBALIDE IN GINKGO-BILOBA LEAF EXTRACTS AND PHYTOPHARMACEUTICALS [J].