Initial binding of Shiga toxin-producing Escherichia coli to host cells and subsequent induction of actin rearrangements depend on filamentous EspA-containing surface appendages

被引:128
作者
Ebel, F [1 ]
Podzadel, T
Rohde, M
Kresse, AU
Krämer, S
Deibel, C
Guzmán, CA
Chakraborty, T
机构
[1] Univ Giessen, Inst Med Mikrobiol, D-35392 Giessen, Germany
[2] GBF Natl Res Ctr Biotechnol, Div Microbiol, D-38124 Braunschweig, Germany
关键词
D O I
10.1046/j.1365-2958.1998.01046.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Shiga toxin-producing Escherichia coli (STEC) induce so-called attaching and effacing lesions that enable the tight adherence of these pathogens to the gut epithelium. All of the genes necessary for this process are present in the locus of enterocyte effacement, which encodes a type III secretion system, the secreted Esp proteins and the surface protein intimin. In this study we sequenced the espA gene of STEC, generated and characterized a corresponding deletion mutant and raised EspA-specific monoclonal antibodies to analyse the functional role of this protein during infection. EspA was detected in often filament-like structures decorating all bacteria that had attached to Hela cells. These appendages were especially prominent on bacteria that had not yet induced the formation of actin pedestals, indicating that they mediate the initial contact of STEC to their target cells. Consistently, a deletion of the espA gene completely abolished the capacity of such STEC mutants to bind to HeLa cells and to induce actin rearrangements. Surface appendages similar to those described in this study are also formed by Pseudomonas syringae and may represent a structural element common to many bacterial pathogens that deliver proteins into their target cells via a type ill secretion system.
引用
收藏
页码:147 / 161
页数:15
相关论文
共 32 条
[1]   Characterization of two virulence proteins secreted by rabbit enteropathogenic Escherichia coli, EspA and EspB, whose maximal expression is sensitive to host body temperature [J].
Abe, P ;
Kenny, B ;
Stein, M ;
Finlay, BB .
INFECTION AND IMMUNITY, 1997, 65 (09) :3547-3555
[2]   CYTOSKELETAL REARRANGEMENTS AND THE FUNCTIONAL-ROLE OF T-PLASTIN DURING ENTRY OF SHIGELLA-FLEXNERI INTO HELA-CELLS [J].
ADAM, T ;
ARPIN, M ;
PREVOST, MC ;
GOUNON, P ;
SANSONETTI, PJ .
JOURNAL OF CELL BIOLOGY, 1995, 129 (02) :367-381
[3]   The Yersinia Yop virulon: A bacterial system for subverting eukaryotic cells [J].
Cornelis, GR ;
WolfWatz, H .
MOLECULAR MICROBIOLOGY, 1997, 23 (05) :861-867
[4]  
DEANNYSTOC EA, 1997, INFECT IMMUN, V65, P1841
[5]   EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein [J].
Deibel, C ;
Krämer, S ;
Chakraborty, T ;
Ebel, F .
MOLECULAR MICROBIOLOGY, 1998, 28 (03) :463-474
[6]   Interactions between enteropathogenic Escherichia coli and host epithelial cells [J].
Donnenberg, MS ;
Kaper, JB ;
Finlay, BB .
TRENDS IN MICROBIOLOGY, 1997, 5 (03) :109-114
[7]   A 2ND CHROMOSOMAL GENE NECESSARY FOR INTIMATE ATTACHMENT OF ENTEROPATHOGENIC ESCHERICHIA-COLI TO EPITHELIAL-CELLS [J].
DONNENBERG, MS ;
YU, J ;
KAPER, JB .
JOURNAL OF BACTERIOLOGY, 1993, 175 (15) :4670-4680
[8]   Temperature- and medium-dependent secretion of proteins by Shiga toxin-producing Escherichia coli [J].
Ebel, F ;
Deibel, C ;
Kresse, AU ;
Guzman, CA ;
Chakraborty, T .
INFECTION AND IMMUNITY, 1996, 64 (11) :4472-4479
[9]   MORPHOLOGICAL AND CYTOSKELETAL CHANGES IN EPITHELIAL-CELLS OCCUR IMMEDIATELY UPON INTERACTION WITH SALMONELLA-TYPHIMURIUM GROWN UNDER LOW-OXYGEN CONDITIONS [J].
FRANCIS, CL ;
STARNBACH, MN ;
FALKOW, S .
MOLECULAR MICROBIOLOGY, 1992, 6 (21) :3077-3087
[10]   AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI [J].
GIRON, JA ;
HO, ASY ;
SCHOOLNIK, GK .
SCIENCE, 1991, 254 (5032) :710-713