Cell-free chemoenzymatic starch synthesis from carbon dioxide

被引：572

作者：

Cai, Tao ^{[1
,2
]}

Sun, Hongbing ^{[1
,2
]}

Qiao, Jing ^{[1
,2
]}

Zhu, Leilei ^{[2
,3
]}

Zhang, Fan ^{[1
,2
]}

Zhang, Jie ^{[2
,3
]}

Tang, Zijing ^{[2
,3
]}

Wei, Xinlei ^{[2
,3
]}

Yang, Jiangang ^{[2
,3
]}

Yuan, Qianqian ^{[2
,4
]}

Wang, Wangyin ^{[5
]}

Yang, Xue ^{[2
,4
]}

Chu, Huanyu ^{[2
,4
]}

Wang, Qian ^{[2
,4
]}

You, Chun ^{[2
,3
]}

Ma, Hongwu ^{[2
,4
]}

Sun, Yuanxia ^{[2
,3
]}

Li, Yin ^{[1
,2
]}

Li, Can ^{[5
]}

Jiang, Huifeng ^{[2
,4
]}

Wang, Qinhong ^{[1
,2
,4
]}

Ma, Yanhe ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Dept Strateg & Integrat Res, Tianjin 300308, Peoples R China

[2] Natl Ctr Technol Innovat Synthet Biol, Tianjin 300308, Peoples R China

[3] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China

[4] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, CAS Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China

[5] Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

来源：

SCIENCE | 2021年 / 373卷 / 6562期

关键词：

ADP-GLUCOSE PYROPHOSPHORYLASE; SITE-DIRECTED MUTAGENESIS; ESCHERICHIA-COLI; TRIOSEPHOSPHATE ISOMERASE; ENZYMATIC-SYNTHESIS; HIGH-YIELD; FIXATION; DESIGN; GLYCOLALDEHYDE; BIOCHEMISTRY;

D O I：

10.1126/science.abh4049

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Starches, a storage form of carbohydrates, are a major source of calories in the human diet and a primary feedstock for bioindustry. We report a chemical-biochemical hybrid pathway for starch synthesis from carbon dioxide (CO2) and hydrogen in a cell-free system. The artificial starch anabolic pathway (ASAP), consisting of 11 core reactions, was drafted by computational pathway design, established through modular assembly and substitution, and optimized by protein engineering of three bottleneck-associated enzymes. In a chemoenzymatic system with spatial and temporal segregation, ASAP, driven by hydrogen, converts CO2 to starch at a rate of 22 nanomoles of CO2 per minute per milligram of total catalyst, an similar to 8.5-fold higher rate than starch synthesis in maize. This approach opens the way toward future chemo-biohybrid starch synthesis from CO2.

引用

页码：1523 / +

页数：59

共 83 条

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