A mammalian protein with specific demethylase activity for mCpG DNA

被引：504

作者：

Bhattacharya, SK ^{[1
]}

Ramchandani, S ^{[1
]}

Cervoni, N ^{[1
]}

Szyf, M ^{[1
]}

机构：

[1] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada

来源：

NATURE | 1999年 / 397卷 / 6720期

关键词：

D O I：

10.1038/17533

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

引用

页码：579 / 583

页数：5

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