Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes

被引：100

作者：

Baer, A ^{[1
]}

Bode, J ^{[1
]}

机构：

[1] German Res Inst Biotechnol, Gesell Biotechnol Forschung mbH, GBF, RDIF Epigenet Regulat, D-38124 Braunschweig, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 05期

关键词：

D O I：

10.1016/S0958-1669(00)00248-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.

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收藏

页码：473 / 480

页数：8

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