Genome-wide RNAi analysis of growth and viability in Drosophila cells

被引:557
作者
Boutros, M
Kiger, AA
Armknecht, S
Kerr, K
Hild, M
Koch, B
Haas, SA
Paro, R
Perrimon, N [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
[3] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany
[4] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
关键词
D O I
10.1126/science.1091266
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.
引用
收藏
页码:832 / 835
页数:4
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