Rapid actin-based plasticity in dendritic spines

被引：746

作者：

Fischer, M ^{[1
]}

Kaech, S ^{[1
]}

Knutti, D ^{[1
]}

Matus, A ^{[1
]}

机构：

[1] Friedrich Miescher Inst, CH-4002 Basel, Switzerland

来源：

NEURON | 1998年 / 20卷 / 05期

关键词：

D O I：

10.1016/S0896-6273(00)80467-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occured within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.

引用

页码：847 / 854

页数：8

共 37 条

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