Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice

被引:275
作者
Dymecki, SM
机构
[1] Department of Embryology, Carnegie Institution of Washington, Baltimore
关键词
D O I
10.1073/pnas.93.12.6191
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Site-specific recombinases are being developed as tools for ''in vivo'' genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites, Here it is demonstrated that Pip recombinase can effectively mediate site-specific excisional recombination in mouse embryonic stem cells, in differentiating embryonal carcinoma cells, and in transgenic mice, Broad Flp expression is compatible with normal development, suggesting that Pip can be used to catalyze recombination in most cell types. These properties indicate that Flp can be exploited to make prescribed alterations in the mouse genome.
引用
收藏
页码:6191 / 6196
页数:6
相关论文
共 42 条
[1]  
ABREMSKI K, 1984, J BIOL CHEM, V259, P1509
[2]   RECOMBINATION WITHIN THE YEAST PLASMID 2-MU CIRCLE IS SITE-SPECIFIC [J].
BROACH, JR ;
GUARASCIO, VR ;
JAYARAM, M .
CELL, 1982, 29 (01) :227-234
[3]   EXPRESSION OF THE DROSOPHILA GOOSEBERRY LOCUS DEFINES A SUBSET OF NEUROBLAST LINEAGES IN THE CENTRAL-NERVOUS-SYSTEM [J].
BUENZOW, DE ;
HOLMGREN, R .
DEVELOPMENTAL BIOLOGY, 1995, 170 (02) :338-349
[4]  
BURRELL H, 1995, EPICENTRE FORUM, V2, P4
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]  
CHOU TB, 1992, GENETICS, V131, P643
[7]   THE FLP PROTEIN OF THE YEAST 2-MU-PLASMID - EXPRESSION OF A EUKARYOTIC GENETIC-RECOMBINATION SYSTEM IN ESCHERICHIA-COLI [J].
COX, MM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (14) :4223-4227
[8]   A modular set of Flp, FRT and lacZ fusion vectors for manipulating genes by site-specific recombination [J].
Dymecki, SM .
GENE, 1996, 171 (02) :197-201
[9]   SONIC-HEDGEHOG, A MEMBER OF A FAMILY OF PUTATIVE SIGNALING MOLECULES, IS IMPLICATED IN THE REGULATION OF CNS POLARITY [J].
ECHELARD, Y ;
EPSTEIN, DJ ;
STJACQUES, B ;
SHEN, L ;
MOHLER, J ;
MCMAHON, JA ;
MCMAHON, AP .
CELL, 1993, 75 (07) :1417-1430
[10]  
ECHELARD Y, 1994, DEVELOPMENT, V120, P2213