A rapid and sensitive assay for histone acetyl-transferase activity

被引：45

作者：

Ait-Si-Ali, S ^{[1
]}

Ramirez, S ^{[1
]}

Robin, P ^{[1
]}

Trouche, D ^{[1
]}

Harel-Bellan, A ^{[1
]}

机构：

[1] CNRS, UPR 9079, Lab Oncogenese Differenciat & Transduct Signal, F-94801 Villejuif, France

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 16期

关键词：

D O I：

10.1093/nar/26.16.3869

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [C-14]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.

引用

页码：3869 / 3870

页数：2

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