Identification and properties of a novel intracellular (mitochondrial) ATP-sensitive potassium channel in brain

被引：240

作者：

Bajgar, R ^{[1
]}

Seetharaman, S ^{[1
]}

Kowaltowski, AJ ^{[1
]}

Garlid, KD ^{[1
]}

Paucek, P ^{[1
]}

机构：

[1] Oregon Hlth Sci Univ, OGI Sch Sci & Engn, Dept Biochem & Mol Biol, Beaverton, OR 97006 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 36期

关键词：

D O I：

10.1074/jbc.M103320200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protection of heart against ischemia-reperfusion injury by ischemic preconditioning and K-ATP channel openers is known to involve the mitochondrial ATP-sensitive K+ channel (mitoK(ATP)). Brain is also protected by ischemic preconditioning and K-ATP channel openers, and it has been suggested that mitoK(ATP) may also play a key role in brain protection. However, it is not known whether mitoK(ATP) exists in brain mitochondria, and, if so, whether its properties are similar to or different from those of heart mitoK(ATP). We report partial purification and reconstitution of a new mitoK(ATP) from rat brain mitochondria. We measured K+ flux in proteoliposomes and found that brain mitoK(ATP) is regulated by the same ligands as those that regulate mitoK(ATP) from heart and liver. We also examined the effects of opening and closing mitoK(ATP) on brain mitochondrial respiration, and we estimated the amount of mitoK(ATP) by means of green fluorescence probe BODIPY-FL-glyburide labeling of the sulfonylurea receptor of mitoK(ATP) from brain and liver. Three independent methods indicate that brain mitochondria contain six to seven times more mitoK(ATP) per milligram of mitochondrial protein than liver or heart.

引用

页码：33369 / 33374

页数：6

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