Molecular characterization of cDNA for phospholipase A2-activating protein

被引：12

作者：

Chopra, AK ^{[1
]}

Ribardo, DA

Wood, TG

Prusak, DJ

Xu, XJ

Peterson, JW

机构：

[1] Univ Texas, Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA

[2] Univ Texas, Med Branch, Ctr Mol Sci & Human Biol Chem & Genet, Galveston, TX 77555 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1444卷 / 01期

关键词：

phospholipase A(2)-activating protein cDNA; phospholipase A(2)-activating protein; cDNA cloning; sequencing; and expression; reverse transcriptase polymerase chain reaction; 3 '-rapid amplification of cDNA ends; anti-sense plap oligonucleotide;

D O I：

10.1016/S0167-4781(98)00249-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phospholipase A(2)-activating protein (PLAP) cDNA was cloned and sequenced from a human monocyte cDNA library, and expressed as a histidine-tagged fusion protein. The DNA-deduced aa sequence of human PLAP was 80,826 Da; however, SDS-PAGE analysis revealed a 72-74 kDa protein which matched the size of native PLAP from human monocytes. Anti-sense plap oligonucleotide blocked cholera toxin-induced release of H-3-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A(2) activity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：125 / 130

页数：6

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