Determination of plasma ET-1 by RIA -: Risks and limitations

被引：3

作者：

Baumgartner-Parzer, SM

Nowotny, P

Wagner, O

Waldhäusl, W

机构：

[1] Univ Vienna, Dept Internal Med 3, Div Endocrinol & Metab, A-1090 Vienna, Austria

[2] Univ Leipzig, Inst Clin Chem & Pathobiochem, D-7010 Leipzig, Germany

来源：

HORMONE AND METABOLIC RESEARCH | 1998年 / 30卷 / 10期

关键词：

endothelin-1; plasma concentration; radioimmunoassay; quality control;

D O I：

10.1055/s-2007-978948

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Plasma concentrations of the vasoconstrictor peptide endothelin-1 (ET-1) were determined in nonobese healthy subjects as well as in unspecified patients using three different commercially available radioimmunoassays (RIAs) and the respective recommended prepurification procedures. Depending on the antibody and extraction procedure applied different ET-1 concentrations were measured in identical plasma samples. Thus, this study clearly documents that ET-1 concentrations measured in plasma samples represent apparent concentrations which have to be interpreted with caution.

引用

页码：633 / 635

页数：3

共 11 条

[1] PRESENCE OF IMMUNOREACTIVE ENDOTHELIN IN HUMAN-PLASMA [J].

ANDO, K ;

HIRATA, Y ;

SHICHIRI, M ;

EMORI, T ;

MARUMO, F .

FEBS LETTERS, 1989, 245 (1-2) :164-166

[2]

CERNACEK P, 1989, BIOCHEM BIOPH RES CO, V137, P317

[3]

KANNO K, 1991, J CARDIOVASC PHAR S7, V17, P475

[4]

KOYAMA H, 1989, LANCET, V1, P991

[5] INCREASED PLASMA-LEVELS OF ENDOTHELIN-1 IN PATIENTS WITH HYPERTHYROIDISM [J].

LETIZIA, C ;

CENTANNI, M ;

CESAREO, R ;

DECIOCCHIS, A ;

CERCI, S ;

SCURO, L ;

FUMAROLA, A ;

DAMBROSIO, C ;

SCAVO, D .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1995, 44 (10) :1239-1242

[6]

SAITO Y, 1990, NEW ENGL J MED, V322, P205

[7] PLASMA ENDOTHELIN LEVELS IN HYPERTENSION AND CHRONIC-RENAL-FAILURE [J].

SHICHIRI, M ;

HIRATA, Y ;

ANDO, K ;

EMORI, T ;

OHTA, K ;

KIMOTO, S ;

OGURA, M ;

INOUE, A ;

MARUMO, F .

HYPERTENSION, 1990, 15 (05) :493-496

[8] ELEVATED PLASMA ENDOTHELIN IN PATIENTS WITH DIABETES-MELLITUS [J].

TAKAHASHI, K ;

GHATEI, MA ;

LAM, HC ;

OHALLORAN, DJ ;

BLOOM, SR .

DIABETOLOGIA, 1990, 33 (05) :306-310

[9] EFFECT OF ENDOTHELIN-1 IN MAN [J].

VIERHAPPER, H ;

WAGNER, O ;

NOWOTNY, P ;

WALDHAUSL, W .

CIRCULATION, 1990, 81 (04) :1415-1418

[10] PLASMA-CONCENTRATIONS OF ENDOTHELIN IN MAN - ARTERIOVENOUS DIFFERENCES AND RELEASE DURING VENOUS STASIS [J].

WAGNER, OF ;

NOWOTNY, P ;

VIERHAPPER, H ;

WALDHAUSL, W .

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1990, 20 (05) :502-505

← 1 2 →