Real-time cell assays of phospholipase A2s using fluorogenic phospholipids

被引：6

作者：

Manna, Debasis ^{[1
]}

Cho, Wonhwa ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Chicago, IL 60607 USA

来源：

LIPIDOMICS AND BIOACTIVE LIPIDS: LIPIDS AND CELL SIGNALING | 2007年 / 434卷

关键词：

D O I：

10.1016/S0076-6879(07)34002-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase A(2)s (PLA(2)s) are a superfamily of enzymes involved in production of a wide variety of lipid mediators, including arachidonic acid, lysophospholipids, platelet activation factor, and eicosanoids. Fluorescence-based, real-time cellular activity assays for PLA(2)s have been developed as a tool for studying the function and spatiotemporal regulation of PLA(2)s. Recent progress in fluorogenic phospholipid design, genetic methods, and multiphoton multichannel microscopy allows simultaneous and continuous measurement of cellular localization and activity of PLA(2) in an isoform-selective manner. These assays should aid in elucidating the physiological roles and regulatory mechanisms of diverse PLA(2)

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页码：15 / +

页数：14

相关论文

共 31 条

[1] Group V secretory phospholipase A2 translocates to the phagosome after zymosan stimulation of mouse peritoneal macrophages and regulates phagocytosis [J].

Balestrieri, B ;

Hsu, VW ;

Gilbert, H ;

Leslie, CC ;

Han, WK ;

Bonventre, JV ;

Arm, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (10) :6691-6698

[2] Calcium-independent phospholipase A2 and apoptosis [J].

Balsinde, Jesus ;

Perez, Rebeca ;

Balboa, Maria A. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2006, 1761 (11) :1344-1350

[3] Insulin secretory responses and phospholipid composition of pancreatic islets from mice that do not express Group VIA phospholipase A2 and effects of metabolic stress on glucose homeostasis [J].

Bao, Shunzhong ;

Song, Haowei ;

Wohltmann, Mary ;

Ramanadham, Sasanka ;

Jin, Wu ;

Bohrer, Alan ;

Turk, John .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (30) :20958-20973

[4] Male mice that do not express group VIA phospholipase A2 produce spermatozoa with impaired motility and have greatly reduced fertility [J].

Bao, SZ ;

Miller, DJ ;

Ma, ZM ;

Wohltmann, M ;

Eng, G ;

Ramanadham, S ;

Moley, K ;

Turk, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (37) :38194-38200

[5] Enzyme activity - it's all about image [J].

Baruch, A ;

Jeffery, DA ;

Bogyo, M .

TRENDS IN CELL BIOLOGY, 2004, 14 (01) :29-35

[6] Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells [J].

Bingham, CO ;

Fijneman, RJA ;

Friend, DS ;

Goddeau, RP ;

Rogers, RA ;

Austen, KF ;

Arm, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (44) :31476-31484

[7] Reduced fertility and postischaemic brain injury in mice deficient in cytosolic phospholipase A(2) [J].

Bonventre, JV ;

Huang, ZH ;

Taheri, MR ;

OLeary, E ;

Li, E ;

Moskowitz, MA ;

Sapirstein, A .

NATURE, 1997, 390 (6660) :622-625

[8] Seeing is believing:: Real-time cellular activity assay for phospholipase A2 [J].

Cho, Wonhwa .

ACS CHEMICAL BIOLOGY, 2006, 1 (02) :65-66

[9] A NOVEL ARACHIDONIC ACID-SELECTIVE CYTOSOLIC PLA2 CONTAINS A CA2+-DEPENDENT TRANSLOCATION DOMAIN WITH HOMOLOGY TO PKC AND GAP [J].

CLARK, JD ;

LIN, LL ;

KRIZ, RW ;

RAMESHA, CS ;

SULTZMAN, LA ;

LIN, AY ;

MILONA, N ;

KNOPF, JL .

CELL, 1991, 65 (06) :1043-1051

[10] Intracellular calcium signals regulating cytosolic phospholipase A2 translocation to internal membranes [J].

Evans, JH ;

Spencer, DM ;

Zweifach, A ;

Leslie, CC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (32) :30150-30160

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