Control of G(1) in the developing Drosophila eye: rca1 regulates Cyclin A

In the developing eye of Drosophila melanogaster, cells become synchronized in the G(1) phase of the cell cycle just prior to the onset of cellular differentiation and morphogenesis. In rougher (rux) mutants, cells enter S phase precociously because of ectopic activation of a Cyclin A/Cdk complex in early G(1). This leads to defects in cell fate and pattern formation, and results in abnormalities in the morphology of the adult eye. A screen for dominant suppressors of the rux eye phenotype led to the identification of mutations in cyclin A, string (cdc25), and new cell cycle genes. One of these genes, regulator of cyclin A (real), encodes a novel protein required for both mitotic and meiotic cell cycle progression. rca1 mutants arrest in G(2) of embryonic cell cycle 16 with a phenotype very similar to cyclin A loss of function mutants. Expression of real transgenes in G(1) or in postmitotic neurons promotes Cyclin A protein accumulation and drives cells into S phase in a Cyclin A-dependent fashion.