Biological performances of collagen-based scaffolds tor vascular tissue engineering

被引：153

作者：

Boccafoschi, F

Habermehl, J

Vesentini, S

Mantovani, D ^{[1
]}

机构：

[1] Univ Laval, Lab Biomat & Bioengn, Ste Foy, PQ G1K 7P4, Canada

[2] Univ Piemonte Orientale, Res Ctr Biocompatibil, Human Anat Lab, Novara, Italy

[3] Politecn Milan, Dept Bioengn, I-20133 Milan, Italy

来源：

BIOMATERIALS | 2005年 / 26卷 / 35期

基金：

加拿大自然科学与工程研究理事会;

关键词：

collagen; blood compatibility; endothelial cells; smooth muscle cells; platelet adhesion;

D O I：

10.1016/j.biomaterials.2005.05.052

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Collagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular tissue engineering. In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent haemocompatibility and cell viability performance assays. First, haemoglobin-free, thrombelastography and platelet adhesion tests were performed in order to investigate the blood contact performance. Secondly, specimens were seeded with endothelial cells and smooth muscle cells, and cell viability tests were carried out by MTT and SEM. Results show that neutralized acid-soluble type I collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation. Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：7410 / 7417

页数：8

共 32 条

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