Chronic optical access through a polished and reinforced thinned skull

被引：270

作者：

Drew, Patrick J. ^{[1
,2
,3
]}

Shih, Andy Y. ^{[1
]}

Driscoll, Jonathan D. ^{[1
]}

Knutsen, Per Magne ^{[1
]}

Blinder, Pablo ^{[1
]}

Davalos, Dimitrios ^{[4
]}

Akassoglou, Katerina ^{[4
,5
]}

Tsai, Philbert S. ^{[1
]}

Kleinfeld, David ^{[1
,6
,7
]}

机构：

[1] Univ Calif San Diego, Dept Phys, San Diego, CA 92103 USA

[2] Penn State Univ, Dept Engn Sci & Mech, Ctr Neural Engn, University Pk, PA 16802 USA

[3] Penn State Univ, Dept Neurosurg, University Pk, PA 16802 USA

[4] Univ Calif San Francisco, Gladstone Inst Neurol Dis, San Francisco, CA 94143 USA

[5] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA

[6] Univ Calif San Diego, Grad Program Neurosci, San Diego, CA 92103 USA

[7] Univ Calif San Diego, Ctr Neural Circuits & Behav, San Diego, CA 92103 USA

来源：

NATURE METHODS | 2010年 / 7卷 / 12期

基金：

加拿大健康研究院; 美国国家卫生研究院;

关键词：

LONG-TERM; PENETRATING ARTERIOLES; BRAIN; CORTEX; BLOOD; CELLS; FLUCTUATIONS; MACROPHAGES; ACTIVATION; NEOCORTEX;

D O I：

10.1038/NMETH.1530

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.

引用

页码：981 / U60

页数：6

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