Insertional mutagenesis in zebrafish

被引：33

作者：

Amsterdam, A ^{[1
]}

机构：

[1] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

来源：

DEVELOPMENTAL DYNAMICS | 2003年 / 228卷 / 03期

关键词：

zebrafish; retrovirus; vertebrate genetics; functional genomics; gene-trap;

D O I：

10.1002/dvdy.10381

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Insertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell viability and growth, revealing the diversity of gene products required for the development of this vertebrate. Here, I will review the methodology of this approach and the results to date, as well as other potential ways to use insertional mutagenesis for genetic screens. (C) 2003 Wiley-Liss, Inc.

引用

页码：523 / 534

页数：12

共 59 条

[1] Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development [J].