Localization of cyclooxygenase-2 and regulation of its mRNA expression in gastric ulcers in rats

被引：94

作者：

Takahashi, S ^{[1
]}

Shigeta, JI

Inoue, H

Tanabe, T

Okabe, S

机构：

[1] Kyoto Pharmaceut Univ, Dept Appl Pharmacol, Kyoto 6078414, Japan

[2] Natl Cardiovasc Ctr, Res Inst, Dept Pharmacol, Suita, Osaka 5658565, Japan

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1998年 / 275卷 / 05期

关键词：

prostaglandin; interleukin-1; beta; tumor necrosis factor-alpha; transforming growth factor-beta 1;

D O I：

10.1152/ajpgi.1998.275.5.G1137

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

It has been reported that cyclooxygenase-2 (COX-2) may play a crucial role in gastric ulcer healing. We examined the localization of COX-2 and the regulation of COX-2 mRNA expression in acetic acid ulcers in rats. PGE(2) production was elevated in ulcerated tissue but not in intact tissue. COX-2 mRNA expression was induced in only the ulcerated tissue, and COX-2 protein was found in fibroblasts, monocytes/macrophages, and granulocytes. A selective COX-2 inhibitor inhibited increased PGE(2) production by the ulcerated tissue. Interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and transforming growth factor-beta 1 (TGF-beta 1) mRNAs were also expressed only in the ulcerated tissue. In a culture of isolated ulcer base, blockade of IL-1 beta and TNF-alpha reduced COX-2 mRNA expression and PGE2 production. In contrast, COX-2 mRNA expression and PGE2 production were promoted by prevention of TGF-beta 1 action. These results indicate that COX-2 protein is highly localized in the base of gastric ulcers in rats and that COX-2 mRNA expression might be regulated positively by IL-1 beta and TNF-alpha and negatively by TGF-beta 1.

引用

页码：G1137 / G1145

页数：9

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