Activation-dependent surface expression of LOX-1 in human platelets

被引：153

作者：

Chen, MY

Kakutani, M

Naruko, T

Ueda, M

Narumiya, S

Masaki, T

Sawamura, T ^{[1
]}

机构：

[1] Natl Cardiovasc Ctr, Res Inst, Osaka 5658565, Japan

[2] Kyoto Univ, Fac Med, Dept Pharmacol, Kyoto 606, Japan

[3] Osaka City Univ, Sch Med, Dept Pharmacol, Osaka 5458585, Japan

[4] Osaka City Gen Hosp, Dept Cardiol, Osaka 5340021, Japan

[5] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Mol Pathophysiol, Osaka 5650871, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 282卷 / 01期

关键词：

LOX-1; oxidized LDL; human;

D O I：

10.1006/bbrc.2001.4516

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lectin-like oxidized LDL receptor-1 (LOX-1) was initially identified as an oxidized LDL receptor in aortic endothelial cells. Here we identified LOX-1 mRNA and protein in human platelets in addition to recent findings on the expression in macrophages and smooth muscle cells. The presence of LOX-1 was further confirmed in the megakaryocytic cell lines. Flow cytometric analyses revealed that LOX-1 was exposed on the surface of platelets in an activation-dependent manner. Consistently, the activation-dependent binding of OxLDL to platelets was mostly inhibited by anti-LOX-1 antibody. Immunohistochemistry of the atherosclerotic plaque from a patient with unstable angina pectoris (UAP) revealed accumulation of LOX-1 protein at the site of thrombus, As LOX-1 recognizes and binds activated platelets, exposure of LOX-1 on activated platelets surface might assist thrombosis formation. (C) 2001 Academic Press.

引用

页码：153 / 158

页数：6

共 31 条

[1] Structure and chromosomal assignment of the human lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1) gene [J].