Influenza A virus lacking the NS1 gene replicates in interferon-deficient systems

被引：855

作者：

García-Sastre, A

Egorov, A

Matassov, D

Brandt, S

Levy, DE

Durbin, JE

Palese, P

Muster, T

机构：

[1] CUNY Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

[2] Inst Angew Mikrobiol, A-1190 Vienna, Austria

[3] Univ Vienna, Sch Med, Dept Dermatol, A-1090 Vienna, Austria

[4] NYU, Sch Med, Dept Pathol, New York, NY 10016 USA

[5] Ohio State Univ, Childrens Hosp Res Fdn, Columbus, OH 43205 USA

来源：

VIROLOGY | 1998年 / 252卷 / 02期

基金：

美国国家卫生研究院; 奥地利科学基金会;

关键词：

D O I：

10.1006/viro.1998.9508

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The NS1 protein is the only nonstructural protein encoded by influenza A virus. It has been proposed that the NS1 performs several regulatory functions during the viral replication cycle, including the regulation of synthesis, transport, splicing, and translation of mRNAs. Through the use of reverse genetics, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated. Our results indicate that the NS1 of influenza A virus is an auxiliary (virulence) factor which plays a crucial role in inhibiting interferon-mediated antiviral responses of the host. (C) 1998 Academic Press.

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页码：324 / 330

页数：7

相关论文

共 36 条

[1] THE INTERFERON-STIMULATED GENE 54 K PROMOTER CONTAINS 2 ADJACENT FUNCTIONAL INTERFERON-STIMULATED RESPONSE ELEMENTS OF DIFFERENT STRENGTH, WHICH ACT SYNERGISTICALLY FOR MAXIMAL INTERFERON-ALPHA INDUCIBILITY [J].

BLUYSSEN, HAR ;

VLIETSTRA, RJ ;

VANDERMADE, A ;

TRAPMAN, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (02) :395-402

[2] Recombinant respiratory syncytial virus from which the entire SH gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse [J].

Bukreyev, A ;

Whitehead, SS ;

Murphy, BR ;

Collins, PL .

JOURNAL OF VIROLOGY, 1997, 71 (12) :8973-8982

[3] INFLUENZA-VIRUS NS1 PROTEIN ENHANCES THE RATE OF TRANSLATION INITIATION OF VIRAL MESSENGER-RNAS [J].

DELALUNA, S ;

FORTES, P ;

BELOSO, A ;

ORTIN, J .

JOURNAL OF VIROLOGY, 1995, 69 (04) :2427-2433

[4] Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein [J].

Delenda, C ;

Hausmann, S ;

Garcin, D ;

Kolakofsky, D .

VIROLOGY, 1997, 228 (01) :55-62

[5] HOMOZYGOUS DELETION OF THE ALPHA-INTERFERON AND BETA-1-INTERFERON GENES IN HUMAN-LEUKEMIA AND DERIVED CELL-LINES [J].

DIAZ, MO ;

ZIEMIN, S ;

LEBEAU, MM ;

PITHA, P ;

SMITH, SD ;

CHILCOTE, RR ;

ROWLEY, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5259-5263

[6] Targeted disruption of the mouse STAT1 results in compromised innate immunity to viral disease [J].

Durbin, JE ;

Hackenmiller, R ;

Simon, MC ;

Levy, DE .

CELL, 1996, 84 (03) :443-450

[7] Transfectant influenza A viruses with long deletions in the NS1 protein grow efficiently in vero cells [J].

Egorov, A ;

Brandt, S ;

Sereinig, S ;

Romanova, J ;

Ferko, B ;

Katinger, D ;

Grassauer, A ;

Alexandrova, G ;

Katinger, H ;

Muster, T .

JOURNAL OF VIROLOGY, 1998, 72 (08) :6437-6441

[8]

Egorov A Iu, 1994, Vopr Virusol, V39, P201

[9] INFLUENZA-VIRUS NS1 PROTEIN STIMULATES TRANSLATION OF THE M1 PROTEIN [J].

ENAMI, K ;

SATO, TA ;

NAKADA, S ;

ENAMI, M .

JOURNAL OF VIROLOGY, 1994, 68 (03) :1432-1437

[10] HIGH-EFFICIENCY FORMATION OF INFLUENZA-VIRUS TRANSFECTANTS [J].

ENAMI, M ;

PALESE, P .

JOURNAL OF VIROLOGY, 1991, 65 (05) :2711-2713

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