学术探索
学术期刊
新闻热点
数据分析
智能评审

S100 proteins interact with the N-terminal domain of MDM2

被引：38
作者：
van Dieck, Jan [1 ]
Lum, Jenifer K. [1 ]
Teufel, Daniel P. [1 ]
Fersht, Alan R. [1 ]
机构：
[1] MRC Ctr Prot Engn, Cambridge CB2 0QH, England
来源：
FEBS LETTERS | 2010年 / 584卷 / 15期
基金：
英国医学研究理事会;
关键词：
S100; p53; MDM2; Ternary complex; Protein-protein interaction; CALCIUM-BINDING PROTEIN; TUMOR-SUPPRESSOR PROTEIN; TRANSCRIPTIONAL ACTIVITY; P53; FAMILY; OLIGOMERIZATION; METASTASIS; MODULATION; TARGETS; CANCER;
D O I：
10.1016/j.febslet.2010.06.024
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
S100 proteins interact with the transactivation domain and the C-terminus of p53. Further, S100B has been shown to interact with MDM2, a central negative regulator of p53. Here, we show that S100B bound directly to the folded N-terminal domain of MDM2 (residues 2-125) by size exclusion chromatography and surface plasmon resonance experiments. This interaction with MDM2 (2-125) is a general feature of S100 proteins; S100A1, S100A2, S100A4 and S100A6 also interact with MDM2 (2-125). These interactions with S100 proteins do not result in a ternary complex with MDM2 (2-125) and p53. Instead, we observe the ability of a subset of S100 proteins to disrupt the extent of MDM2-mediated p53 ubiquitylation in vitro. Structured summary: MINT-7905256: MDM2 (uniprotkb:Q00987) binds (MI:0407) to s100A6 (uniprotkb:P06703) by surface plasmon resonance (MI:0107) MINT-7905063: MDM2 (uniprotkb:Q00987) and s100A1 (uniprotkb:P23297) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905376: s100A4 (uniprotkb:P26447) and MDM2 (uniprotkb:Q00987) physically interact (MI :0915) by competition binding (MI:0405) MINT-7905130: s100A6 (uniprotkb:P06703) and MDM2 (uniprotkb:Q00987) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905207: s100A6 (uniprotkb:P06703) and p53 (uniprotkb:P04637) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905043: s100B (uniprotkb:P04271) and MDM2 (uniprotkb:Q00987) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905196: p53 (uniprotkb:P04637) and s100A4 (uniprotkb:P26447) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905358: p53 (uniprotkb:P04637) and s100A4 (uniprotkb:P26447) physically interact (MI:0915) by fluorescence polarization spectroscopy (MI:0053) MINT-7905220: MDM2 (uniprotkb:Q00987) binds (MI:0407) to s100B (uniprotkb:P04271) by surface plasmon resonance (MI:0107) MINT-7905104: s100A4 (uniprotkb:P26447) and MDM2 (uniprotkb:Q00987) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905229: MDM2 (uniprotkb:Q00987) binds (MI:0407) to s100A1 (uniprotkb:P23297) by surface plasmon resonance (MI:0107) MINT-7905317, MINT-7905162: s100B (uniprotkb:P04271) and p53 (uniprotkb:P04637) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905238: MDM2 (uniprotkb:Q00987) binds (MI:0407) to s100A2 (uniprotkb:P29034) by surface plasmon resonance (MI:0107) MINT-7905174, M1NT-7905308: s100A1 (uniprotkb:P23297) and p53 (uniprotkb:P04637) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905247: MDM2 (uniprotkb:Q00987) binds (MI:0407) to s100A4 (uniprotkb:P26447) by surface plasmon resonance (MI:0107) MINT-7905090: s100A2 (uniprotkb:P29034) and MDM2 (uniprotkb:Q00987) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905142, MINT-7905326: MDM2 (uniprotkb:Q00987) and p53 (uniprotkb:P04637) bind (MI:0407) by molecular sieving (MI:0071) MINT-7905185, MINT-7905347: s100A2 (uniprotkb:P29034) and p53 (uniprotkb:P04637) bind (MI:0407) by molecular sieving (MI:0071) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3269 / 3274
页数：6
相关论文
共 23 条
[1]  
Ambartsumian NS, 1996, ONCOGENE, V13, P1621
[2]   Effects of common cancer mutations on stability and DNA binding of full-length p53 compared with isolated core domains [J].
Ang, Hwee Ching ;
Joerger, Andreas C. ;
Mayer, Sebastian ;
Fersht, Alan R. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (31) :21934-21941
[3]   The luminescence immunoassay S-100: a sensitive test to measure circulating S-100B: its prognostic value in malignant melanoma [J].
Bonfrer, JMG ;
Korse, CM ;
Nieweg, OE ;
Rankin, EM .
BRITISH JOURNAL OF CANCER, 1998, 77 (12) :2210-2214
[4]  
Davies MPA, 1996, ONCOGENE, V13, P1631
[5]   Intracellular and extracellular roles of s100 proteins [J].
Donato, R .
MICROSCOPY RESEARCH AND TECHNIQUE, 2003, 60 (06) :540-551
[6]   Members of the S100 family bind p53 in two distinct ways [J].
Fernandez-Fernandez, Maria Rosario ;
Rutherford, Trevor J. ;
Fersht, Alan R. .
PROTEIN SCIENCE, 2008, 17 (10) :1663-1670
[7]   Proteins of the S100 family regulate the oligomerization of p53 tumor suppressor [J].
Fernandez-Fernandez, MR ;
Veprintsev, DB ;
Fersht, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (13) :4735-4740
[8]   Cooperative regulation of p53 by modulation of ternary complex formation with CBP/p300 and HDM2 [J].
Ferreon, Josephine C. ;
Lee, Chul Won ;
Arai, Munehito ;
Martinez-Yamout, Maria A. ;
Dyson, H. Jane ;
Wright, Peter E. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (16) :6591-6596
[9]   Binding of S100 proteins to RAGE: An update [J].
Leclerc, Estelle ;
Fritz, Guenter ;
Vetter, Stefan W. ;
Heizmann, Claus W. .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2009, 1793 (06) :993-1007
[10]   Inhibition of p53 transcriptional activity by the S100B calcium-binding protein [J].
Lin, J ;
Blake, M ;
Tang, C ;
Zimmer, D ;
Rustandi, RR ;
Weber, DJ ;
Carrier, F .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (37) :35037-35041
123