A simple method for delivering morpholino antisense oligos into the cytoplasm of cells

被引：83

作者：

Partridge, M ^{[1
]}

Vincent, A ^{[1
]}

Matthews, P ^{[1
]}

Puma, J ^{[1
]}

Stein, D ^{[1
]}

Summerton, J ^{[1
]}

机构：

[1] ANTIVIRALS INC,CORVALLIS,OR 97333

来源：

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT | 1996年 / 6卷 / 03期

关键词：

D O I：

10.1089/oli.1.1996.6.169

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a simple and effective means for delivering Morpholino antisense oligos into the cytosol of cultured anchorage-dependent animal cells, This method, referred to as scrape-loading, is carried out in a matter of seconds, uses a common inexpensive laboratory implement, and has minimal detrimental impact on the cells, Using this delivery method, a Morpholino oligo present at 0.1 mu M and 1 mu M in the extracellular medium inhibited its targeted genetic sequence within cultured Hela cells at levels of 56% and 85%, respectively. Lack of inhibition by two control Morpholino oligos at concentrations up to 3 mu M indicates good sequence specificity by this structural type, Also described is a test system for simple, rapid, and sensitive quantitation of antisense activity in cultured cells.

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页码：169 / 175

页数：7

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