Detection of single-nucleotide polymorphisms with the WAVE™ DNA fragment analysis system

被引：119

作者：

Kuklin, A ^{[1
]}

Munson, K ^{[1
]}

Gjerde, D ^{[1
]}

Haefele, R ^{[1
]}

Taylor, P ^{[1
]}

机构：

[1] Transgen Inc, San Jose, CA 95131 USA

来源：

GENETIC TESTING | 1997年 / 1卷 / 03期

关键词：

D O I：

10.1089/gte.1997.1.201

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

As the Human Genome Project is generating an avalanche of genetic information, molecular researchers and clinical practitioners are setting new criteria for evaluation of the links between newly discovered gene mutations and human disorders. These requirements necessitate the development of highly accurate and yet rapid automated systems for genetic testing. We describe the detection of a single nucleotide polymorphism (SNP) on the human Y chromosome with the fully automated, sensitive, and rapid system WAVE(TM) designed for DNA fragment analysis. This new technology, based on temperature-modulated liquid chromatography and a high-resolution matrix, offers new dimensions to the molecular biology research. The versatility of the WAVE(TM) makes the equipment a universal molecular biology stations for clinical and research facilities. The key aspects to setting the operating parameters are discussed.

引用

页码：201 / 206

页数：6

共 18 条

[1] GENETIC-DISEASE DETECTION AND DNA AMPLIFICATION USING CLONED THERMOSTABLE LIGASE [J].