共 52 条
Mitochondrial release of AIF and EndoG requires caspase activation downstream of Bax/Bak-mediated permeabilization
被引:359
作者:

Arnoult, D
论文数: 0 引用数: 0
h-index: 0
机构: NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA

Gaume, B
论文数: 0 引用数: 0
h-index: 0
机构: NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA

Karbowski, M
论文数: 0 引用数: 0
h-index: 0
机构: NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA

Sharpe, JC
论文数: 0 引用数: 0
h-index: 0
机构: NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA

Cecconi, F
论文数: 0 引用数: 0
h-index: 0
机构: NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA

Youle, RJ
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA
机构:
[1] NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA
[2] Univ Roma Tor Vergata, Dept Biol, I-00173 Rome, Italy
关键词:
AIF;
cytochrome c;
EndoG;
HtrA2;
Omi;
Smac;
Diablo;
D O I:
10.1093/emboj/cdg423
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Mitochondrial outer-membrane permeabilization by pro-apoptotic Bcl-2 family members plays a crucial role in apoptosis induction. However, whether this directly causes the release of the different mitochondrial apoptogenic factors simultaneously is currently unknown. Here we report that in cells or with isolated mitochondria, pro-apoptotic Bcl-2 proteins cause the release of cytochrome c, Smac/Diablo and HtrA2/Omi but not endonuclease G (EndoG) and apoptosis-inducing factor (AIF). In cells treated with Bax/Bak-dependent pro-apoptotic drugs, neither the caspase inhibitor zVAD-fmk nor loss of Apaf-1 affected the efflux of cytochrome c, Smac/Diablo and HtrA2/Omi, but both prevented the release of EndoG and AIF. Our findings identify the mitochondrial response to pro-apoptotic stimuli as a selective process leading to a hierarchical ordering of the effectors involved in cell death induction. Moreover, as in Caenorhabditis elegans, EndoG and AIF act downstream of caspase activation. Thus EndoG and AIF seem to define a 'caspase-dependent' mitochondria-initiated apoptotic DNA degradation pathway that is conserved between mammals and nematodes.
引用
收藏
页码:4385 / 4399
页数:15
相关论文
共 52 条
[1]
Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2
[J].
Adrain, C
;
Creagh, EM
;
Martin, SJ
.
EMBO JOURNAL,
2001, 20 (23)
:6627-6636

Adrain, C
论文数: 0 引用数: 0
h-index: 0
机构:
Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland

Creagh, EM
论文数: 0 引用数: 0
h-index: 0
机构:
Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland

Martin, SJ
论文数: 0 引用数: 0
h-index: 0
机构:
Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland Trinity Coll Dublin, Smurfit Inst, Dept Genet, Mol Cell Biol Lab, Dublin 2, Ireland
[2]
Bax oligomerization is required for channel-forming activity in liposomes and to trigger cytochrome c release from mitochondria
[J].
Antonsson, B
;
Montessuit, S
;
Lauper, S
;
Eskes, R
;
Martinou, JC
.
BIOCHEMICAL JOURNAL,
2000, 345
:271-278

Antonsson, B
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Montessuit, S
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Lauper, S
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Eskes, R
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Martinou, JC
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland
[3]
Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuli
[J].
Arnoult, D
;
Parone, P
;
Martinou, JC
;
Antonsson, B
;
Estaquier, J
;
Ameisen, JC
.
JOURNAL OF CELL BIOLOGY,
2002, 159 (06)
:923-929

Arnoult, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France

Parone, P
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France

Martinou, JC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France

Antonsson, B
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France

Estaquier, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France

Ameisen, JC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 07, INSERM, Inst Natl Sante & Rech Med, EMI U 9922,CHU Bichat,IFR02,AP HP, F-75018 Paris, France
[4]
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development
[J].
Cecconi, F
;
Alvarez-Bolado, G
;
Meyer, BI
;
Roth, KA
;
Gruss, P
.
CELL,
1998, 94 (06)
:727-737

Cecconi, F
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany

Alvarez-Bolado, G
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany

Meyer, BI
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany

Roth, KA
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany

Gruss, P
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany
[5]
Mitochondria as the central control point of apoptosis
[J].
Desagher, S
;
Martinou, JC
.
TRENDS IN CELL BIOLOGY,
2000, 10 (09)
:369-377

Desagher, S
论文数: 0 引用数: 0
h-index: 0
机构: CCIPE, CNRS 9023, UPR, F-34094 Montpellier 5, France

Martinou, JC
论文数: 0 引用数: 0
h-index: 0
机构: CCIPE, CNRS 9023, UPR, F-34094 Montpellier 5, France
[6]
Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis
[J].
Desagher, S
;
Osen-Sand, A
;
Nichols, A
;
Eskes, R
;
Montessuit, S
;
Lauper, S
;
Maundrell, K
;
Antonsson, B
;
Martinou, JC
.
JOURNAL OF CELL BIOLOGY,
1999, 144 (05)
:891-901

Desagher, S
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Osen-Sand, A
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Nichols, A
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Eskes, R
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Montessuit, S
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Lauper, S
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Maundrell, K
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Antonsson, B
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Martinou, JC
论文数: 0 引用数: 0
h-index: 0
机构:
Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland Ares Serono Int SA, Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland
[7]
Cytochrome c release from isolated rat liver mitochondria can occur independently of outer-membrane rupture:: possible role of contact sites
[J].
Doran, E
;
Halestrap, AP
.
BIOCHEMICAL JOURNAL,
2000, 348 (02)
:343-350

Doran, E
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England

Halestrap, AP
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England
[8]
Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition
[J].
Du, CY
;
Fang, M
;
Li, YC
;
Li, L
;
Wang, XD
.
CELL,
2000, 102 (01)
:33-42

Du, CY
论文数: 0 引用数: 0
h-index: 0
机构: Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA

Fang, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA

Li, YC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA

Li, L
论文数: 0 引用数: 0
h-index: 0
机构: Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA

Wang, XD
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75235 USA
[9]
Bax-induced cytochrome C release from mitochondria is independent of the permeability transition pore but highly dependent on Mg2+ ions
[J].
Eskes, R
;
Antonsson, B
;
Osen-Sand, A
;
Montessuit, S
;
Richter, C
;
Sadoul, R
;
Mazzei, G
;
Nichols, A
;
Martinou, JC
.
JOURNAL OF CELL BIOLOGY,
1998, 143 (01)
:217-224

Eskes, R
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Antonsson, B
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Osen-Sand, A
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Montessuit, S
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Richter, C
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Sadoul, R
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Mazzei, G
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Nichols, A
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland

Martinou, JC
论文数: 0 引用数: 0
h-index: 0
机构: Serono Pharmaceut Res Inst, CH-1228 Geneva, Switzerland
[10]
Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL
[J].
Finucane, DM
;
Bossy-Wetzel, E
;
Waterhouse, NJ
;
Cotter, TG
;
Green, DR
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
1999, 274 (04)
:2225-2233

Finucane, DM
论文数: 0 引用数: 0
h-index: 0
机构: La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA

Bossy-Wetzel, E
论文数: 0 引用数: 0
h-index: 0
机构: La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA

Waterhouse, NJ
论文数: 0 引用数: 0
h-index: 0
机构: La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA

Cotter, TG
论文数: 0 引用数: 0
h-index: 0
机构: La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA

Green, DR
论文数: 0 引用数: 0
h-index: 0
机构: La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA