Genomic analysis of heat-shock factor targets in Drosophila -: art. no. R63

被引：70

作者：

Birch-Machin, I

Gao, S

Huen, D

McGirr, R

White, RAH

Russell, S

机构：

[1] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England

[2] Univ Cambridge, Dept Anat, Cambridge CB2 3EH, England

来源：

GENOME BIOLOGY | 2005年 / 6卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1186/gb-2005-6-7-r63

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.

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页数：16

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