Analysis of efficiency of two-photon versus single-photon absorption for fluorescence generation in biological objects

被引：36

作者：

Brakenhoff, GJ

Muller, M

Ghauharali, RI

机构：

[1] Department of Molecular Cytology, University of Amsterdam, 1098 SM, Amsterdam

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1996年 / 183卷

关键词：

fluorescence; confocal microscopy; two-photon absorption imaging; biological damage; photobleaching; sectioning;

D O I：

10.1046/j.1365-2818.1996.870647.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Starting from basic absorption cross-section values and breakdown limitations on possible field strengths it is shown that the total exposure required for equivalent fluorescence generation by single-photon absorption (SPA) is at least an order of magnitude lower than by two-photon absorption (TPA). The difference is such that TPA may in fact in many cases offer no advantage over SPA with respect to the damage induced during fluorescence imaging of biological materials.

引用

页码：140 / 144

页数：5

共 17 条

[1]

Boyd R. W., 2019, Nonlinear Optics, V4th

[2] Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system [J].