Dynamics of the COPII coat with GTP and stable analogues

被引：236

作者：

Antonny, B

Madden, D

Hamamoto, S

Orci, L

Schekman, R ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Univ Geneva, Dept Morphol, Ctr Med Univ, CH-1211 Geneva 4, Switzerland

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 06期

关键词：

D O I：

10.1038/35078500

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed an assay to monitor the assembly of the COPII coat onto liposomes in real time. We show that with Sar1pGTP bound to liposomes, a single round of assembly and disassembly of the COPII coat lasts a few seconds. The two large COPII complexes Sec23/24p and Sec13/31p bind almost instantaneously (in less than 1 s) to Sar1pGTP-doped liposomes. This binding is followed by a fast (less than 10 s) disassembly due to a 10-fold acceleration of the GTPase-activating protein activity of Sec23/24p by the Sec13/31p complex. Experiments with the phosphate analogue BeFx suggest that Sec23/24p provides residues directly involved in GTP hydrolysis on Sar1p.

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页码：531 / 537

页数：7

相关论文

共 30 条

[1] N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange [J].

Antonny, B ;

BeraudDufour, S ;

Chardin, P ;

Chabre, M .

BIOCHEMISTRY, 1997, 36 (15) :4675-4684

[2] The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly [J].

Aridor, M ;

Fish, KN ;

Bannykh, S ;

Weissman, J ;

Roberts, TH ;

Lippincott-Schwartz, J ;

Balch, WE .

JOURNAL OF CELL BIOLOGY, 2001, 152 (01) :213-229

[3] Cargo selection by the COPII budding machinery during export from the ER [J].

Aridor, M ;

Weissman, J ;

Bannykh, S ;

Nuoffer, C ;

Balch, WE .

JOURNAL OF CELL BIOLOGY, 1998, 141 (01) :61-70

[4]

BARLOWE C, 1993, J BIOL CHEM, V268, P873

[5] COPII - A MEMBRANE COAT FORMED BY SEC PROTEINS THAT DRIVE VESICLE BUDDING FROM THE ENDOPLASMIC-RETICULUM [J].

BARLOWE, C ;

ORCI, L ;

YEUNG, T ;

HOSOBUCHI, M ;

HAMAMOTO, S ;

SALAMA, N ;

REXACH, MF ;

RAVAZZOLA, M ;

AMHERDT, M ;

SCHEKMAN, R .

CELL, 1994, 77 (06) :895-907

[6] SEC12 ENCODES A GUANINE-NUCLEOTIDE-EXCHANGE FACTOR ESSENTIAL FOR TRANSPORT VESICLE BUDDING FROM THE ER [J].

BARLOWE, C ;

SCHEKMAN, R .

NATURE, 1993, 365 (6444) :347-349

[7] Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors [J].

Bremser, M ;

Nickel, W ;

Schweikert, M ;

Ravazzola, M ;

Amherdt, M ;

Hughes, CA ;

Söllner, TH ;

Rothman, JE ;

Wieland, FT .

CELL, 1999, 96 (04) :495-506

[8] ALUMINOFLUORIDE AND BERYLLOFLUORIDE COMPLEXES - NEW PHOSPHATE ANALOGS IN ENZYMOLOGY [J].

CHABRE, M .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (01) :6-10

[9] THE ARF1 GTPASE-ACTIVATING PROTEIN - ZINC-FINGER MOTIF AND GOLGI-COMPLEX LOCALIZATION [J].

CUKIERMAN, E ;

HUBER, I ;

ROTMAN, M ;

CASSEL, D .

SCIENCE, 1995, 270 (5244) :1999-2002

[10] TRYPTOPHAN-W207 IN TRANSDUCIN T-ALPHA IS THE FLUORESCENCE SENSOR OF THE G-PROTEIN ACTIVATION SWITCH AND IS INVOLVED IN THE EFFECTOR-BINDING [J].

FAUROBERT, E ;

OTTOBRUC, A ;

CHARDIN, P ;

CHABRE, M .

EMBO JOURNAL, 1993, 12 (11) :4191-4198