The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-β-D-thiogalactopyranoside exclusion and spectinomycin sensitivity

被引：53

作者：

Bohn, C ^{[1
]}

Bouloc, P ^{[1
]}

机构：

[1] Univ Paris Sud, Inst Genet & Microbiol, Lab Reseaux Regulat, CNRS,URA 2225, F-91405 Orsay, France

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 22期

关键词：

D O I：

10.1128/JB.180.22.6072-6075.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype, cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.

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页码：6072 / 6075

页数：4

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