New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

被引：16

作者：

An, ZQ

Farman, ML

Budde, A

Taura, S

Leong, SA

机构：

[1] UNIV WISCONSIN,DEPT PLANT PATHOL,MADISON,WI 53706

[2] USDA ARS,PLANT DIS RESISTANCE RES UNIT,MADISON,WI 53706

来源：

GENE | 1996年 / 176卷 / 1-2期

关键词：

ascomycetes; basidiomycetes; transformant; promoter;

D O I：

10.1016/0378-1119(96)00225-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/mu g linear DNA and U. maydis at up to 25 transformants/mu g circular DNA for integrative transformation. In addition, 2800 transformants/mu g DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.

引用

页码：93 / 96

页数：4

共 22 条

[1] A COSMID VECTOR FOR SYSTEMATIC CHROMOSOME WALKING [J].