Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive amplicon profiling of breast cancers - A new approach for the molecular analysis of paraffin-embedded cancer tissue

被引：70

作者：

Daigo, Y

Chin, SF

Gorringe, KL

Bobrow, LG

Ponder, BAJ

Pharoah, PDP

Caldas, C

机构：

[1] Cambridge Inst Med Res, Wellcome Trust Ctr Mol Mech Dis,, Dept Oncol, Cambridge CB2 2XY, England

[2] Univ Cambridge, Strangeways Res Labs, Cambridge CB2 2XY, England

[3] Addenbrookes Hosp, Cambridge Breast Unit, Cambridge CB2 2XY, England

[4] Cambridge Inst Med Res, Wellcome Trust Ctr Mol Mech Dis, Dept Pathol, Cambridge CB2 2XY, England

来源：

AMERICAN JOURNAL OF PATHOLOGY | 2001年 / 158卷 / 05期

关键词：

D O I：

10.1016/S0002-9440(10)64118-1

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We have developed a protocol for degenerate oligonuclotide-primed-polymerase chain reaction-based array comparative genomic hybridization (array CGH) that, when combined with a laser microdissection technique, allows the analysis of cancer cell populations isolated from routine, formalin-axed, paraffin-embedded tissue samples. Comparison of copy number changes detected by degenerate oligonucleotide-primed-polymerase chain reaction-based array CGH to those detected by conventional array CGH or fluorescence ill situ hybridization, demonstrated that amplifications can be reliably detected. Using a genomic microarray containing 57 oncogenes, we screened a total of 28 breast cancer samples and obtained a detailed amplicon profile that is the most comprehensive to date in human breast cancer. The array CGH method described here will allow the genetic analysis of paraffin-embedded human cancer materials for example in the context of clinical trials.

引用

页码：1623 / 1631

页数：9

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