An open and closed case for all polymerases

被引:282
作者
Doublié, S
Sawaya, MR
Ellenberger, T
机构
[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[2] Univ Vermont, Markey Ctr Mol Genet, Dept Microbiol & Mol Genet, Burlington, VT 05405 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/S0969-2126(99)80017-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The recently determined structures of HIV-1 reverse transcriptase and Taq DNA polymerase in complex with DNA primer-template and an incoming nucleotide have shown that a large conformational change configures the polymerase active site for nucleotidyl transfer. The structure of reverse transcriptase in the catalytic complex will open the path to the rational design of novel nucleoside analog inhibitors of viral replication.
引用
收藏
页码:R31 / R35
页数:5
相关论文
共 22 条
[1]   A minor groove binding track in reverse transcriptase [J].
Bebenek, K ;
Beard, WA ;
Darden, TA ;
Li, LP ;
Prasad, R ;
Luxon, BA ;
Gorenstein, DG ;
Wilson, SH ;
Kunkel, TA .
NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) :194-197
[2]   STRUCTURAL BASIS FOR THE 3'-5' EXONUCLEASE ACTIVITY OF ESCHERICHIA-COLI DNA-POLYMERASE-I - A 2 METAL-ION MECHANISM [J].
BEESE, LS ;
STEITZ, TA .
EMBO JOURNAL, 1991, 10 (01) :25-33
[3]   AN ATTEMPT TO UNIFY THE STRUCTURE OF POLYMERASES [J].
DELARUE, M ;
POCH, O ;
TORDO, N ;
MORAS, D ;
ARGOS, P .
PROTEIN ENGINEERING, 1990, 3 (06) :461-467
[4]   Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution [J].
Doublié, S ;
Tabor, S ;
Long, AM ;
Richardson, CC ;
Ellenberger, T .
NATURE, 1998, 391 (6664) :251-258
[5]   Structure of Taq polymerase with DNA at the polymerase active site [J].
Eom, SH ;
Wang, JM ;
Steitz, TA .
NATURE, 1996, 382 (6588) :278-281
[6]   MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MOLONEY MURINE LEUKEMIA-VIRUS REVERSE-TRANSCRIPTASE [J].
GEORGIADIS, MM ;
JESSEN, SM ;
OGATA, CM ;
TELESNITSKY, A ;
GOFF, SP ;
HENDRICKSON, WA .
STRUCTURE, 1995, 3 (09) :879-892
[7]   Structure of a covalently trapped catalytic complex of HIV-I reverse transcriptase: Implications for drug resistance [J].
Huang, HF ;
Chopra, R ;
Verdine, GL ;
Harrison, SC .
SCIENCE, 1998, 282 (5394) :1669-1675
[8]   CRYSTAL-STRUCTURE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE COMPLEXED WITH DOUBLE-STRANDED DNA AT 3.0 ANGSTROM RESOLUTION SHOWS BENT DNA [J].
JACOBOMOLINA, A ;
DING, JP ;
NANNI, RG ;
CLARK, AD ;
LU, XD ;
TANTILLO, C ;
WILLIAMS, RL ;
KAMER, G ;
FERRIS, AL ;
CLARK, P ;
HIZI, A ;
HUGHES, SH ;
ARNOLD, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) :6320-6324
[9]   Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal [J].
Kiefer, JR ;
Mao, C ;
Braman, JC ;
Beese, LS .
NATURE, 1998, 391 (6664) :304-307
[10]   Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 angstrom resolution [J].
Kiefer, JR ;
Mao, C ;
Hansen, CJ ;
Basehore, SL ;
Hogrefe, HH ;
Braman, JC ;
Beese, LS .
STRUCTURE, 1997, 5 (01) :95-108