Making and reading microarrays

被引：475

作者：

Cheung, VG ^{[1
]}

Morley, M

Aguilar, F

Massimi, A

Kucherlapati, R

Childs, G

机构：

[1] Univ Penn, Dept Pediat, Philadelphia, PA 19104 USA

[2] Yeshiva Univ Albert Einstein Coll Med, Dept Mol Genet, Bronx, NY 10461 USA

来源：

NATURE GENETICS | 1999年 / 21卷 / Suppl 1期

关键词：

D O I：

10.1038/4439

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

There are a variety of options for making microarrays and obtaining microarray data. Here, we describe the building and the use of two microarray facilities in academic settings. In addition to specifying technical detail, we comment on the advantages and disadvantages of components and approaches, and provide a protocol for hybridization. The fact that we are now making and using microarrays to answer biological questions demonstrates that the technology can be implemented in university environment.

引用

收藏

页码：15 / 19

页数：5

相关论文

共 14 条

[1] ESTABLISHING A HUMAN TRANSCRIPT MAP [J].

BOGUSKI, MS ;

SCHULER, GD .

NATURE GENETICS, 1995, 10 (04) :369-371

[2] Options available - from start to finish - for obtaining expression data by microarray [J].

Bowtell, DDL .

NATURE GENETICS, 1999, 21 (Suppl 1) :25-32

[3] RATES OF FORMATION AND THERMAL STABILITIES OF RNA-DNA AND DNA-DNA DUPLEXES AT HIGH-CONCENTRATIONS OF FORMAMIDE [J].

CASEY, J ;

DAVIDSON, N .

NUCLEIC ACIDS RESEARCH, 1977, 4 (05) :1539-1552

[4] Linkage-disequilibrium mapping without genotyping [J].

Cheung, VG ;

Gregg, JP ;

Gogolin-Ewens, KJ ;

Bandong, J ;

Stanley, CA ;

Baker, L ;

Higgins, MJ ;

Nowak, NJ ;

Shows, TB ;

Ewens, WJ ;

Spielman, RS .

NATURE GENETICS, 1998, 18 (03) :225-230

[5] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[6]

DeRisi J, 1996, NAT GENET, V14, P457

[7] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[8] Expression profiling using cDNA microarrays [J].

Duggan, DJ ;

Bittner, M ;

Chen, YD ;

Meltzer, P ;

Trent, JM .

NATURE GENETICS, 1999, 21 (Suppl 1) :10-14

[9] Data management and analysis for gene expression arrays [J].

Ermolaeva, O ;

Rastogi, M ;

Pruitt, KD ;

Schuler, GD ;

Bittner, ML ;

Chen, YD ;

Simon, R ;

Meltzer, P ;

Trent, JM ;

Boguski, MS .

NATURE GENETICS, 1998, 20 (01) :19-23

[10]

Sambrook J., 1989, MOL CLONING