Use of generic fast gradient liquid chromatography tandem mass spectroscopy in quantitative bioanalysis

被引：60

作者：

Ayrton, J ^{[1
]}

Dear, GJ ^{[1
]}

Leavens, WJ ^{[1
]}

Mallett, DN ^{[1
]}

Plumb, RS ^{[1
]}

机构：

[1] Glaxo Wellcome Res & Dev Ltd, Bioanal & Drug Metab Div, Int Dev, Ware SG12 0DJ, Herts, England

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 709卷 / 02期

关键词：

quantitative analysis;

D O I：

10.1016/S0378-4347(98)00074-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Short narrow analytical HPLC columns have been used successfully with high linear flow-rates and combined with mass spectrometric detection to produce a generic approach to quantitative bioanalysis. The approach has been used to validate several assays in the low ng/ml region and an example is given in this paper. When combined with a simple solid-phase extraction process the need for complicated, time consuming method development has been removed for the majority of pharmaceutical compounds. The approach takes advantage of not only the extra selectivity of the MS-MS detector but the excellent resolution and peak shape produced by gradient elution. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：243 / 254

页数：12

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