Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania

被引：8

作者：

Guimaraes, TMPD

deToledo, VDCP

daCosta, CA

daCosta, RT

Genaro, O

Williams, P

Mayrink, W

机构：

[1] BIOBRAS SA,DEPT PESQUISAS,BELO HORIZONT,MG,BRAZIL

[2] UNIV FED MINAS GERAIS,DEPT PARASITOL,INST CIENCIAS BIOL,BELO HORIZONT,MG,BRAZIL

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1996年 / 91卷 / 01期

关键词：

Leishmania; glycoproteins; monoclonal antibodies;

D O I：

10.1590/S0074-02761996000100010

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of gamma-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th-1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.

引用

页码：63 / 70

页数：8

共 47 条

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