Expression of laminin β1 in hippocampi of patients with intractable epilepsy

被引：22

作者：

Wu, Yuan ^{[1
,2
]}

Wang, Xue-feng ^{[1
]}

Mo, Xue-an ^{[2
]}

Sun, Hong-bin ^{[3
]}

Li, Jin-mei ^{[1
]}

Zeng, yan ^{[1
]}

Lin, Tao ^{[1
]}

Yuan, Jie ^{[1
]}

Xi, Zhi-qin ^{[1
]}

Zhu, Xi ^{[1
]}

Zheng, Jin-ou ^{[2
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China

[2] Guangxi Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Neurol, Nanning 530022, Peoples R China

[3] Sichuan Prov Hosp, Dept Neurol, Chengdu 610072, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2008年 / 443卷 / 03期

基金：

美国国家卫生研究院;

关键词：

intractable epilepsy; laminin beta 1; FQ-PCR; immunohistochemistry; immunofluorescence; western blotting;

D O I：

10.1016/j.neulet.2008.07.080

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated laminin beta 1 expression in the hippocampi of patients with intractable epilepsy and explored the role of laminin beta 1 in the pathogenesis of this condition. Fluorescence quantitative PCR, immunofluorescence, immunohistochemistry and Western blotting were used to measure laminin beta 1 expression in surgically removed hippocampi of patients with intractable epilepsy, and the results were compared with control hippocarnpi. Fluorescence quantitative PCR showed increased. expression of laminin beta 1 mRNA in patient hippocampi compared with control tissues. Immunohistochemical staining demonstrated that laminin beta 1 protein expression was significantly increased in patient hippocampi, and immunofluorescence microscopy showed accumulation of laminin beta 1 in the cell membrane and cytoplasm of patient hippocampi. These findings were confirmed by Western blotting of protein preparations from patient hippocampi. Elevated expression of laminin beta 1 mRNA and protein in the hippocampus suggests that laminin beta 1 may play a role in the development of epileptic seizures in patients with intractable epilepsy. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：160 / 164

页数：5

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