Discovery and analysis of antisense oligonucleotide activity in cell culture

被引：24

作者：

Baker, BF ^{[1
]}

Condon, TP ^{[1
]}

Koller, E ^{[1
]}

McKay, RA ^{[1
]}

Siwkowski, AM ^{[1
]}

Vickers, TA ^{[1
]}

Monia, BP ^{[1
]}

机构：

[1] ISIS Pharmaceut, Carlsbad, CA 92008 USA

来源：

METHODS | 2001年 / 23卷 / 02期

关键词：

D O I：

10.1006/meth.2000.1120

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for dissection of gene function in molecular cell biology (Koller, E., Gaarde, W. A., and Monia, B. P. (2000) Trends Pharm. Sci., 21, 142-148), and validation of gene targets in animal models (Crooke, S. T. (1998) Biotechnol. Gen. Eng. Rev. 15, 121-157), as well as a means for therapeutic treatment of human diseases (Bennett, C. F. (1999) Exp. Opin. Invest. Drugs 8, 237-253). An important step toward usage of ASOs in the described applications is identification of an active ASO. This article describes the underlying basis and means for achieving this goal in cell culture. (C) 2001 Academic Press.

引用

页码：191 / 198

页数：8

共 56 条

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