Cytoskeletal remodelling and slow dynamics in the living cell

被引：391

作者：

Bursac, P

Lenormand, G

Fabry, B

Oliver, M

Weitz, DA

Viasnoff, V

Butler, JP

Fredberg, JJ

机构：

[1] Harvard Univ, Sch Publ Hlth, Physiol Program, Boston, MA 02115 USA

[2] Univ Erlangen Nurnberg, Inst Biomed Technol, D-91054 Erlangen, Germany

[3] Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA

[4] Harvard Univ, Rowland Inst Sci, Cambridge, MA 02142 USA

[5] Tohoku Univ, Sch Med, Dept Geriatr & Resp Med, Sendai, Miyagi 9808574, Japan

来源：

NATURE MATERIALS | 2005年 / 4卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmat1404

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Forced and spontaneous motions of microbeads tightly bound to the cytoskeleton (CSK) of human muscle cells were analyzed. HASM cells in passages 6-7 were serum-deprived for 24 h; serum deprivation arrests the cell cylce in the G1/G00 phases. It was observed that these spontaneous bead motions reflecting nanoscale CSK arrangements depended on both the approach to glass transition and energy release due to ATP hydrolysis. The results show that the trapping, intermittency, and approach to kinetic arrest are mesoscale features of CSK protein-protein interaction that link integrative phenotypic functions to the molecular events.

引用

页码：557 / 561

页数：5

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