In situ observation of streptavidin-biotin binding on an immunoassay well surface using an atomic force microscope

被引：63

作者：

Allen, S ^{[1
]}

Davies, J ^{[1
]}

Dawkes, AC ^{[1
]}

Davies, MC ^{[1
]}

Edwards, JC ^{[1
]}

Parker, MC ^{[1
]}

Roberts, CJ ^{[1
]}

Sefton, J ^{[1
]}

Tendler, SJB ^{[1
]}

Williams, PM ^{[1
]}

机构：

[1] JOHNSON & JOHNSON CLIN DIAGNOST LTD,POLLARDS WOOD LABS,CHALFONT ST GILES HP8 4SP,BUCKS,ENGLAND

来源：

FEBS LETTERS | 1996年 / 390卷 / 02期

关键词：

atomic force microscopy; ELISA; force-distance curve; molecular interaction; streptavidin-biotin;

D O I：

10.1016/0014-5793(96)00651-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions, We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions between individual streptavidin and biotin molecules on such wells. This was achieved by functionalising an AFM probe with biotin and monitoring the adhesive forces between the probe and a streptavidin coated immunoassay well. The results demonstrate that the AFM may be employed as an analytical tool to study the interactions between biomolecules involved in immunoassay systems.

引用

页码：161 / 164

页数：4

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