Directed evolution of biocatalysts

被引：292

作者：

Arnold, FH ^{[1
]}

Volkov, AA ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn 210-41, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1999年 / 3卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(99)80010-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement. Shuffling of homologous genes offers a new way to utilize natural diversity in the evolution of novel catalysts.

引用

页码：54 / 59

页数：6

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