SWI/SNF chromatin remodeling requires changes in DNA topology

被引：100

作者：

Gavin, I ^{[1
]}

Horn, PJ ^{[1
]}

Peterson, CL ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Biol, Program Mol Med, Worcester, MA 01605 USA

来源：

MOLECULAR CELL | 2001年 / 7卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(01)00158-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ySWI/SNF complex belongs to a family of enzymes that use the energy of ATP hydrolysis to remodel chromatin structure. Here we examine the role of DNA topology in the mechanism of ySWI/SNF remodeling. We find that the ability of ySWI/SNF to enhance accessibility of nucleosomal DNA is nearly eliminated when DNA topology is constrained in small circular nucleosomal arrays and that this inhibition can be alleviated by topoisomerases. Furthermore, we demonstrate that remodeling of these substrates does not require dramatic histone octamer movements or displacement. Our results suggest a model in which ySWI/SNF remodels nucleosomes by using the energy of ATP hydrolysis to drive local changes in DNA twist.

引用

页码：97 / 104

页数：8

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