A vector for expressing foreign genes in the brains and hearts of transgenic mice

被引：317

作者：

Borchelt, DR

Davis, J

Fischer, M

Lee, MK

Slunt, HH

Ratovitsky, T

Regard, J

Copeland, NG

Jenkins, NA

Sisodia, SS

Price, DL

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,NEUROPATHOL LAB,BALTIMORE,MD 21205

[4] UNIV ZURICH,INST MOL BIOL,ABT 1,CH-8093 ZURICH,SWITZERLAND

[5] NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1996年 / 13卷 / 06期

关键词：

expression vector; transgenic; brain;

D O I：

10.1016/S1050-3862(96)00167-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5' intronic, and 3' untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin 1 demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain. (C) 1996 Elsevier Science B.V.

引用

页码：159 / 163

页数：5

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