Enhancing immune responses using suicidal DNA vaccines

被引：192

作者：

Berglund, P

Smerdou, C

Fleeton, MN

Tubulekas, I

Liljeström, P

机构：

[1] Karolinska Inst, Microbiol & Tumorbiol Ctr, S-17177 Stockholm, Sweden

[2] Karolinska Inst, Novum, Dept Biosci, S-14187 Huddinge, Sweden

[3] Swedish Inst Infect Dis Control, Dept Vaccine Res, S-10521 Stockholm, Sweden

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 06期

关键词：

applied immunology; influenza; Semliki Forest virus;

D O I：

10.1038/nbt0698-562

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a DNA vaccine strategy that allows antigens to be produced in vivo in the context of an alphaviral replicon. Mice immunized with such vectors developed humoral and cellular immune responses at higher levels than mice that received a conventional DNA vaccine vector. Immunized animals acquired protective immunity to lethal influenza challenge. Compared with traditional DNA vaccine strategies in which vectors are persistent and the expression constitutive, the expression mediated by the alphaviral vector was transient and lytic. As a result, biosafety risks such as chromosomal integration, and the induction of immunological tolerance, could be circumvented.

引用

页码：562 / 565

页数：4

共 43 条

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