Assembly of the neutrophil respiratory burst oxidase:: A direct interaction between p67PHOX and cytochrome b558

被引：96

作者：

Dang, PMC ^{[1
]}

Cross, AR ^{[1
]}

Babior, BM ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 06期

关键词：

D O I：

10.1073/pnas.061029698

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Activation of the phagocyte NADPH oxidase complex requires the assembly of the cytosolic factors p47(PHOX), P67(PHOX), P40(PHOX), and Rad or Rac2, with the membrane-bound cytochrome b(558) Whereas the interaction of p47(PHOX) with cytochrome b(558) is well established, an interaction between p67(PHOX) and cytochrome b(558) has never been investigated. We report here a direct interaction between p67(PHOX) and cytochrome b(558) First, labeled p67(PHOX) recognizes a 91-kDa band in specific: granules from a normal patient but not from a cytochrome b(558)-deficient patient. Second, p67(PHOX) binds to cytochrome b(558) that has been bound to nitrocellulose. Third, GTP-p67(PHOX) bound to glutathione agarose is able to pull down cytochrome b(558). Rac1-GTP or Rac1-GDP increased the binding of p67(PHOX) to cytochrome b(558), suggesting that at least one of the oxidase-retated functions of Rad is to promote the interaction between p67(PHOX) and cytochrome b(558).

引用

页码：3001 / 3005

页数：5

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