Recent advances in large-scale transposon mutagenesis

被引：62

作者：

Hamer, L ^{[1
]}

DeZwaan, TM ^{[1
]}

Montenegro-Chamorro, MV ^{[1
]}

Frank, SA ^{[1
]}

Hamer, JE ^{[1
]}

机构：

[1] Paradigm Genet Inc, Res Triangle Pk, NC 27709 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(00)00162-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transposons were identified as mobile genetic elements over fifty years ago and subsequently became powerful tools for molecular-genetic studies. Recently, transposon-mutagenesis strategies have been developed to identify essential and pathogenicity-related genes in pathogenic microorganisms. Also, a number of in vitro transposition systems have been used to facilitate genome sequence analysis. Finally, transposon mutagenesis of yeast and complex eukaryotes has provided valuable functional genomic information to complement genome-sequencing projects.

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页码：67 / 73

页数：7

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